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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYT7 Polyclonal antibody (19741-1-AP)

    WB analysis using 19741-1-AP (same clone as 19741-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19741-1-AP (SYT7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19741-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SYT7 Polyclonal antibody (19741-1-AP)

    IHC staining of mouse brain using 19741-1-AP (same clone as 19741-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19741-1-AP (SYT7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19741-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using SYT7 Polyclonal antibody (19741-1-AP)

    IHC staining of mouse cerebellum using 19741-1-AP (same clone as 19741-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19741-1-AP (SYT7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19741-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using SYT7 Polyclonal antibody (19741-1-AP)

    IF Staining of mouse cerebellum using 19741-1-AP (same clone as 19741-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using SYT7 antibody (19741-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19741-1-PBS in a different storage buffer formulation.

SYT7 Polyclonal antibody, PBS Only

SYT7 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 19741-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

IPCA 7, IPCA-7, PCANAP7, Prostate cancer-associated protein 7, Synaptotagmin 7

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19741-1-PBS targets SYT7 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name synaptotagmin VII
Calculated molecular weight 46 kDa
Observed molecular weight42-45 kDa
GenBank accession numberNM_004200
Gene Symbol SYT7
Gene ID (NCBI) 9066
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43581
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SYT7, also named as PCANAP7 and IPCA-7, belongs to the synaptotagmin family. SYT7 may be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis.

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