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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SYT7 Polyclonal antibody

SYT7 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 19741-1-AP

Synonyms

IPCA 7, PCANAP7, Synaptotagmin 7, synaptotagmin VII, SYT VII, SYT7, SytVII

Validation Data Gallery

Applications:
  • WB analysis using 19741-1-AP

    WB analysis using 19741-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19741-1-AP (SYT7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse cerebellum using 19741-1-AP

    IF Staining of mouse cerebellum using 19741-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using SYT7 antibody (19741-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IF detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

19741-1-AP targets SYT7 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name synaptotagmin VII
Calculated molecular weight 46 kDa
Observed molecular weight42 kDa
GenBank accession numberNM_004200
Gene symbol SYT7
Gene ID (NCBI) 9066
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SYT7, also named as PCANAP7 and IPCA-7, belongs to the synaptotagmin family. SYT7 may be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis.

Protocols

Product Specific Protocols
WB protocol for SYT7 antibody 19741-1-APDownload protocol
IF protocol for SYT7 antibody 19741-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols