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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYT2 Polyclonal antibody (21245-1-AP)

    WB analysis using 21245-1-AP (same clone as 21245-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21245-1-AP (SYT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21245-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SYT2 Polyclonal antibody (21245-1-AP)

    WB analysis using 21245-1-AP (same clone as 21245-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21245-1-AP (SYT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21245-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using SYT2 Polyclonal antibody (21245-1-AP)

    IF Staining of mouse cerebellum using 21245-1-AP (same clone as 21245-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using SYT2 antibody (21245-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21245-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using SYT2 Polyclonal antibody (21245-1-AP)

    IF Staining of mouse cerebellum using 21245-1-AP (same clone as 21245-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using SYT2 antibody (21245-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21245-1-PBS in a different storage buffer formulation.

SYT2 Polyclonal antibody, PBS Only

SYT2 Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 21245-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, Indirect ELISA

Synonyms

Synaptotagmin II, Synaptotagmin-2, SytII

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21245-1-PBS targets SYT2 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15744

Product name: Recombinant human SYT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-60 aa of BC100815

Sequence: MRNIFKRNQEPIVAPATTTATMPIGPVDNSTESGGAGESQEDMFAKLKEKLFNEINKIPL

相同性解析による交差性が予測される生物種
Full Name synaptotagmin II
Calculated molecular weight 419 aa, 47 kDa
Observed molecular weight70 kDa
GenBank accession numberBC100815
Gene Symbol SYT2
Gene ID (NCBI) 127833
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N9I0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SYT2 (Synaptotagmin-2) a synaptic vesicle membrane protein, functions as a calcium sensor in vesicular trafficking and exocytosis. SYT2 is also plays a role in dendrite formation by melanocytes (PMID: 23999003). Mutations in SYT2 are associated with myasthenic syndrome, presynaptic, congenital, with or without motor neuropathy. SYT2 mutations cause a complex presynaptic congenital myasthenic syndrome (PMID: 26519543).

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