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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYT2 Polyclonal antibody (21245-1-AP)

    WB analysis using 21245-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21245-1-AP (SYT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SYT2 Polyclonal antibody (21245-1-AP)

    WB analysis using 21245-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21245-1-AP (SYT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using SYT2 Polyclonal antibody (21245-1-AP)

    IF Staining of mouse cerebellum using 21245-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using SYT2 antibody (21245-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using SYT2 Polyclonal antibody (21245-1-AP)

    IF Staining of mouse cerebellum using 21245-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using SYT2 antibody (21245-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SYT2 Polyclonal antibody

SYT2 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 21245-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

Synaptotagmin II, Synaptotagmin-2, SytII

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue, Neuro-2a cells
Positive IF-P detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21245-1-AP targets SYT2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15744

Product name: Recombinant human SYT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-60 aa of BC100815

Sequence: MRNIFKRNQEPIVAPATTTATMPIGPVDNSTESGGAGESQEDMFAKLKEKLFNEINKIPL

相同性解析による交差性が予測される生物種
Full Name synaptotagmin II
Calculated molecular weight 419 aa, 47 kDa
Observed molecular weight70 kDa
GenBank accession numberBC100815
Gene Symbol SYT2
Gene ID (NCBI) 127833
RRIDAB_3085646
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N9I0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SYT2 (Synaptotagmin-2) a synaptic vesicle membrane protein, functions as a calcium sensor in vesicular trafficking and exocytosis. SYT2 is also plays a role in dendrite formation by melanocytes (PMID: 23999003). Mutations in SYT2 are associated with myasthenic syndrome, presynaptic, congenital, with or without motor neuropathy. SYT2 mutations cause a complex presynaptic congenital myasthenic syndrome (PMID: 26519543).

Protocols

Product Specific Protocols
IF protocol for SYT2 antibody 21245-1-APDownload protocol
WB protocol for SYT2 antibody 21245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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