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CoraLite® Plus 488-conjugated SYK Monoclonal antibody
SYK Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
WB
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4C4A12
Cat no : CL488-66721
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | Daudi cells, Ramos cells, Raji cells |
For other applications, we recommend the unconjugated version of this antibody, 66721-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-66721 targets SYK in WB applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SYK fusion protein Ag17654 相同性解析による交差性が予測される生物種 |
| Full Name | spleen tyrosine kinase |
| Calculated molecular weight | 635 aa, 72 kDa |
| Observed molecular weight | 70-75 kDa |
| GenBank accession number | BC001645 |
| Gene symbol | SYK |
| Gene ID (NCBI) | 6850 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. Although SYK has not been linked to a human disease, defective expression of the closely related T-cell tyrosine kinase ZAP-70 has been associated with severe combined immunodeficiency(PMID:11494125). This protein has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 SYK antibody CL488-66721 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
