Validation Data Gallery
Product Information
The ChromoTek SUMO1-Trap Agarose, Kit for Immunoprecipitation consists of an anti-SUMO-Tag NANOBODY®/VHH, which is coupled to agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of SUMO1 proteins from cell extracts of various organisms.
| Description | The SUMO1-Trap Agarose Kit contains SUMO1-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of SUMO1 proteins. • Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis • Works in samples from: mammals, plants, yeast, etc. |
| Applications | IP, Co-IP |
| Specificity/Target | Binds specifically to human SUMO1 (Small Ubiquitin-like Modifier 1). The ChromoTek SUMO1-Trap Agarose will immunoprecipitate both monovalent SUMO1 and SUMO1 chains formed by the process of SUMOylation. In addition to human SUMO1, it will also bind SUMO1 homologs from other mammals. |
| Binding Capacity | 25 ug of SUMO1 protein (~40 kDa) per 25 uL bead slurry |
| Conjugate | Agarose beads; ~90 um (cross-linked 4% agarose beads) |
| Elution buffer | 2x SDS-sample buffer (Lammli), 200 mM glycine pH 2.5 |
| Wash Buffer Compatibility | / |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | / |
| Compatibility with mass spectrometry | The SUMO1-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_3716415 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. DO not freeze! |
Kit components
| Component | Description |
|---|---|
| SUMO1-Trap Agarose | 20 reactions (500 µl) |
| Lysis buffer | Optimized for cytoplasmic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |
Documentation
| SDS |
|---|
| s1tak_SDS_SUMO1-Trap Agarose Kit (EN) |
| Datasheet |
|---|
| SUMO1-Trap Agarose, Kit for Immunoprecipitation Datasheet |