Validation Data Gallery
Product Information
The ChromoTek SUMO-Tag-Trap Magnetic Agarose consists of an anti-SUMO-Tag Nanobody/VHH, which is coupled to magnetic agarose beads. It can be used for the immunoprecipitation of SUMO-tagged proteins from cell extracts of various organisms. The ChromoTek SUMO-Tag-Trap can also be used in conjunction with SUMO proteases such as SenP2 for on-bead digestion of SUMO-Tag fusion proteins to release the protein of interest.
| Description | Immunoprecipitation of SUMO-tagged proteins with anti-SUMO-Tag Nanobody conjugated to magnetic agarose beads. • Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis • Works in samples from: mammals, plants, yeast, etc. |
| Applications | IP, Co-IP |
| Specificity/Target | Binds specifically to all common variants of the SUMO-Tag. The SUMO-Tag is based on Small Ubiquitin-like Modifier (SUMO) proteins of a size of ca. 11 kDa, which are covalently attached to target proteins as a post-translational modification. Fusion of the SUMO-Tag to a protein of interest (POI) may increase expression and solubility of the POI. Also, the SUMO-Tag can be specifically removed by SUMO proteases such as SenP2 without leaving non-native residues behind. At least three SUMO variants are commonly used as SUMO-Tag and are all recognized by the ChromoTek SUMO-Tag-Trap: the yeast SUMO homolog SMT3, the human SUMO3 and SUMOStar, a version of SMT3 resistant to SUMO proteases. Please note that the ChromoTek SUMO-Tag-Trap will also bind non-discriminatorily to endogenous SUMO homologs such as SUMO1, SUMO2 and SUMO3 present in human cells. |
| Binding Capacity | 30 μg of recombinant SUMO-tagged protein (~40 kDa) per 25 μL bead slurry |
| Conjugate | Magnetic Agarose beads; ~40 um (cross-linked 6% magnetic agarose beads) |
| Elution buffer | 2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5 |
| Wash Buffer Compatibility | 1 M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0.0% SDS, 2 M Urea |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | 50 nM |
| Compatibility with mass spectrometry | The SUMO-Tag-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_3668963 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. Do not freeze! |
| Size | 25ul/reactions (eg：20rxns=500ul slurry) |
Documentation
| SDS |
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| sutma_SDS_SUMO-Trap Magnetic Agarose (EN) |
| Datasheet |
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| Protocol SUMO-Tag-Trap Magnetic Agarose (PDF) |