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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STYXL1 Polyclonal antibody (16433-1-AP)

    WB analysis using 16433-1-AP (same clone as 16433-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16433-1-AP (STYXL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16433-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using STYXL1 Polyclonal antibody (16433-1-AP)

    WB analysis using 16433-1-AP (same clone as 16433-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16433-1-AP (STYXL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16433-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using STYXL1 Polyclonal antibody (16433-1-AP)

    IF Staining of HeLa using 16433-1-AP (same clone as 16433-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using STYXL1 antibody (16433-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16433-1-PBS in a different storage buffer formulation.

STYXL1 Polyclonal antibody, PBS Only

STYXL1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 16433-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16433-1-PBS targets STYXL1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9743

Product name: Recombinant human STYXL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-313 aa of BC024035

Sequence: MPGLLLCEPTELYNILNQATKLSRLTDPNYLCLLDVRSKWEYDESHVITALRVKKKNNEYLLPESVDLECVKYCVVYDNNSSTLEILLKDDDDDSDSDGDGKDLVPQAAIEYGRILTRLTHHPVYILKGGYERFSGTYHFLRTQKIIWMPQELDAFQPYPIEIVPGKVFVGNFSQACDPKIQKDLKIKAHVNVSMDTGPFFAGDADKLLHIRIEDSPEAQILPFLRHMCHFIEIHHHLGSVILIFSTQGISRSCAAIIAYLMHSNEQTLQRSWAYVKKCKNNMCPNRGLVSQLLEWEKTILGDSITNIMDPLY

相同性解析による交差性が予測される生物種
Full Name serine/threonine/tyrosine interacting-like 1
Calculated molecular weight 313 aa, 36 kDa
Observed molecular weight25-36 kDa
GenBank accession numberBC024035
Gene Symbol STYXL1
Gene ID (NCBI) 51657
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6J8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

STYXL1, Serine/threonine/tyrosine-interacting-like protein 1, is an inner mitochondrial membrane protein that plays a role in induction of stimulus-dependent apoptosis via the intrinsic apoptotic pathway (PMID: 21262771). STYXL1 catalyzes inactive phosphatase (PMID: 20180778, PMID: 23163895). By binding to G3BP1, STYXL1 inhibits the formation of G3BP1-induced stress granules (PMID: 20180778, PMID: 23163895).

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