Validation Data Gallery
Tested Applications
| Positive WB detected in | NCI-H1299 cells, PC-12 cells, mouse testis tissue, rat testis tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
16433-1-AP targets STYXL1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9743 Product name: Recombinant human STYXL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-313 aa of BC024035 Sequence: MPGLLLCEPTELYNILNQATKLSRLTDPNYLCLLDVRSKWEYDESHVITALRVKKKNNEYLLPESVDLECVKYCVVYDNNSSTLEILLKDDDDDSDSDGDGKDLVPQAAIEYGRILTRLTHHPVYILKGGYERFSGTYHFLRTQKIIWMPQELDAFQPYPIEIVPGKVFVGNFSQACDPKIQKDLKIKAHVNVSMDTGPFFAGDADKLLHIRIEDSPEAQILPFLRHMCHFIEIHHHLGSVILIFSTQGISRSCAAIIAYLMHSNEQTLQRSWAYVKKCKNNMCPNRGLVSQLLEWEKTILGDSITNIMDPLY 相同性解析による交差性が予測される生物種 |
| Full Name | serine/threonine/tyrosine interacting-like 1 |
| Calculated molecular weight | 313 aa, 36 kDa |
| Observed molecular weight | 25-36 kDa |
| GenBank accession number | BC024035 |
| Gene Symbol | STYXL1 |
| Gene ID (NCBI) | 51657 |
| RRID | AB_3085490 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6J8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
STYXL1, Serine/threonine/tyrosine-interacting-like protein 1, is an inner mitochondrial membrane protein that plays a role in induction of stimulus-dependent apoptosis via the intrinsic apoptotic pathway (PMID: 21262771). STYXL1 catalyzes inactive phosphatase (PMID: 20180778, PMID: 23163895). By binding to G3BP1, STYXL1 inhibits the formation of G3BP1-induced stress granules (PMID: 20180778, PMID: 23163895).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for STYXL1 antibody 16433-1-AP | Download protocol |
| WB protocol for STYXL1 antibody 16433-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Sci LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis. | ||
Int Immunopharmacol Linderalactone mitigates diabetic cardiomyopathy in mice via suppressing the MAPK/ATF6 pathway |