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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STUB1 Polyclonal antibody (29751-1-AP)

    WB analysis using 29751-1-AP (same clone as 29751-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29751-1-AP (STUB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29751-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using STUB1 Polyclonal antibody (29751-1-AP)

    WB analysis of HeLa using 29751-1-AP (same clone as 29751-1-PBS)

    WB result of STUB1 antibody (29751-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STUB1 transfected HeLa cells. This data was developed using the same antibody clone with 29751-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using STUB1 Polyclonal antibody (29751-1-AP)

    IHC staining of human placenta using 29751-1-AP (same clone as 29751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29751-1-AP (STUB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29751-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using STUB1 Polyclonal antibody (29751-1-AP)

    IHC staining of human placenta using 29751-1-AP (same clone as 29751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29751-1-AP (STUB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29751-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat lung tissue using STUB1 Polyclonal antibody (29751-1-AP)

    IHC staining of rat lung using 29751-1-AP (same clone as 29751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 29751-1-AP (STUB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29751-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat lung tissue using STUB1 Polyclonal antibody (29751-1-AP)

    IHC staining of rat lung using 29751-1-AP (same clone as 29751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 29751-1-AP (STUB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29751-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

STUB1 Polyclonal antibody, PBS Only

STUB1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29751-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

CHIP, Antigen NY-CO-7, Carboxy terminus of Hsp70-interacting protein, CLL-associated antigen KW-8, E3 ubiquitin-protein ligase CHIP

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29751-1-PBS targets STUB1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31335

Product name: Recombinant human STUB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 73-303 aa of BC007545

Sequence: MQQHEQALADCRRALELDGQSVKAHFFLGQCQLEMESYDEAIANLQRAYSLAKEQRLNFGDDIPSALRIAKKKRWNSIEERRIHQESELHSYLSRLIAAERERELEECQRNHEGDEDDSHVRAQQACIEAKHDKYMADMDELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGITYDRKDIEEHLQRVGHFDPVTRSPLTQEQLIPNLAMKEVIDAFISENGWVEDY

相同性解析による交差性が予測される生物種
Full Name STIP1 homology and U-box containing protein 1
Observed molecular weight35 kDa
GenBank accession numberBC007545
Gene Symbol STUB1
Gene ID (NCBI) 10273
RRIDAB_2923603
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UNE7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

STUB1, also named as CHIP, SDCCAG7, UBOX1 and NY-CO-7, is a E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. STUB1/CHIP modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. It mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. STUB1/CHIP mediates polyubiquitination of CYP3A4.(PMID:10330192) CHIP/STUB1 strongly reduced keratin aggregates through increased degradation of mutant K14. (PMID:20151404) STUB1/CHIP regulates NAD(P)H:quinone oxidoreductase 1 (NQO1) accumulation in aged brain, a process impaired in certain Alzheimer patients.(PMID:21220432 )

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