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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STUB1 Polyclonal antibody (29751-1-AP)

    WB analysis using 29751-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29751-1-AP (STUB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using STUB1 Polyclonal antibody (29751-1-AP)

    WB analysis of HeLa using 29751-1-AP

    WB result of STUB1 antibody (29751-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STUB1 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human placenta tissue using STUB1 Polyclonal antibody (29751-1-AP)

    IHC staining of human placenta using 29751-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29751-1-AP (STUB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using STUB1 Polyclonal antibody (29751-1-AP)

    IHC staining of human placenta using 29751-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29751-1-AP (STUB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat lung tissue using STUB1 Polyclonal antibody (29751-1-AP)

    IHC staining of rat lung using 29751-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 29751-1-AP (STUB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat lung tissue using STUB1 Polyclonal antibody (29751-1-AP)

    IHC staining of rat lung using 29751-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 29751-1-AP (STUB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • KD/KO Validated

STUB1 Polyclonal antibody

STUB1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29751-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CHIP, Antigen NY-CO-7, Carboxy terminus of Hsp70-interacting protein, CLL-associated antigen KW-8, E3 ubiquitin-protein ligase CHIP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, PC-12 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inrat lung tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29751-1-AP targets STUB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STUB1 fusion protein Ag31335 相同性解析による交差性が予測される生物種
Full Name STIP1 homology and U-box containing protein 1
Observed molecular weight35 kDa
GenBank accession numberBC007545
Gene Symbol STUB1
Gene ID (NCBI) 10273
RRIDAB_2923603
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UNE7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STUB1, also named as CHIP, SDCCAG7, UBOX1 and NY-CO-7, is a E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. STUB1/CHIP modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. It mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. STUB1/CHIP mediates polyubiquitination of CYP3A4.(PMID:10330192) CHIP/STUB1 strongly reduced keratin aggregates through increased degradation of mutant K14. (PMID:20151404) STUB1/CHIP regulates NAD(P)H:quinone oxidoreductase 1 (NQO1) accumulation in aged brain, a process impaired in certain Alzheimer patients.(PMID:21220432 )

Protocols

Product Specific Protocols
WB protocol for STUB1 antibody 29751-1-APDownload protocol
IHC protocol for STUB1 antibody 29751-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols