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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated STRAP Monoclonal antibody

STRAP Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5E11F3

Cat no : CL594-66712

Synonyms

MAP activator with WD repeats, MAWD, PT WD, STRAP, UNR interacting protein, UNRIP, WD 40 repeat protein PT WD

Validation Data Gallery

Applications:
  • IF Staining of HEK-293 using CL594-66712

    IF Staining of HEK-293 using CL594-66712

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite®594 STRAP antibody (CL594-66712, Clone: 5E11F3 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy STRAP Ready-To-Use IHC Kit. STRAP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66712-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66712 targets STRAP in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STRAP fusion protein Ag13085 相同性解析による交差性が予測される生物種
Full Name serine/threonine kinase receptor associated protein
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC000162
Gene symbol STRAP
Gene ID (NCBI) 11171
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STRAP (Serine-threonine kinase receptor-associated protein), also known as MAWD, is a ubiquitous WD40 domain protein, common function of which is to provide a suitable scaffold for coordinating multiprotein complex assemblies and thus, regulate a variety of cellular processes like signal transduction, transcriptional regulation, programmed cell death and so on. STRAP plays a role in the cellular distribution of the SMN complex, which is essential for spliceosomal snRNP assembly in the cytoplasm. STRAP inhibits transforming growth factor-beta (TGF-beta) signaling and enhances tumorigenicity via TGF-beta-dependent and -independent mechanisms. STRAP is up-regulated in several cancers and functions as an oncogene. STRAP imparts oncogenic characteristics to cells by promoting ERK and pRb phosphorylation. Moreover, STRAP regulates c-Jun stability by decreasing the ubiquitylation and proteosomal degradation of c-Jun.

Protocols

Product Specific Protocols
IF protocol for CL594 STRAP antibody CL594-66712Download protocol
Standard Protocols
Click here to view our Standard Protocols