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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using STK36 Polyclonal antibody (12559-1-AP)

    WB analysis of mouse testis using 12559-1-AP (same clone as 12559-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12559-1-AP (STK36 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12559-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using STK36 Polyclonal antibody (12559-1-AP)

    IP experiment of mouse testis using 12559-1-AP (same clone as 12559-1-PBS)

    IP result of anti-STK36 (IP:12559-1-AP, 4ug; Detection:12559-1-AP 1:500) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 12559-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STK36 Polyclonal antibody (12559-1-AP)

    IHC staining of human lung cancer using 12559-1-AP (same clone as 12559-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12559-1-AP (STK36 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12559-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using STK36 Polyclonal antibody (12559-1-AP)

    IHC staining of human lung cancer using 12559-1-AP (same clone as 12559-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12559-1-AP (STK36 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12559-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using STK36 Polyclonal antibody (12559-1-AP)

    IHC staining of mouse stomach using 12559-1-AP (same clone as 12559-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12559-1-AP (STK36 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12559-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat stomach tissue using STK36 Polyclonal antibody (12559-1-AP)

    IHC staining of rat stomach using 12559-1-AP (same clone as 12559-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12559-1-AP (STK36 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12559-1-PBS in a different storage buffer formulation.

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STK36 Polyclonal antibody, PBS Only

STK36 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12559-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12559-1-PBS targets STK36 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STK36 fusion protein Ag3279 相同性解析による交差性が予測される生物種
Full Name serine/threonine kinase 36, fused homolog (Drosophila)
Calculated molecular weight 1315 aa, 144 kDa
Observed molecular weight 144 kDa
GenBank accession numberBC026158
Gene Symbol STK36
Gene ID (NCBI) 27148
RRIDAB_2196785
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRP7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

STK36(Serine/threonine-protein kinase 36) is also named as KIAA1278 and belongs to the Ser/Thr protein kinase family. The Serine/threonine protein kinase plays an important role in the sonic hedgehog (Shh) pathway by regulating the activity of GLI transcription factors and controls the activity of the transcriptional regulators GLI1, GLI2 and GLI3 by opposing the effect of SUFU and promoting their nuclear localization. It has 2 isoforms produced by alternative splicing.