Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human prostate tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
12559-1-AP targets STK36 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | STK36 fusion protein Ag3279 相同性解析による交差性が予測される生物種 |
| Full Name | serine/threonine kinase 36, fused homolog (Drosophila) |
| Calculated molecular weight | 1315 aa, 144 kDa |
| Observed molecular weight | 144 kDa |
| GenBank accession number | BC026158 |
| Gene Symbol | STK36 |
| Gene ID (NCBI) | 27148 |
| RRID | AB_2196785 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRP7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
STK36(Serine/threonine-protein kinase 36) is also named as KIAA1278 and belongs to the Ser/Thr protein kinase family. The Serine/threonine protein kinase plays an important role in the sonic hedgehog (Shh) pathway by regulating the activity of GLI transcription factors and controls the activity of the transcriptional regulators GLI1, GLI2 and GLI3 by opposing the effect of SUFU and promoting their nuclear localization. It has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for STK36 antibody 12559-1-AP | Download protocol |
| IHC protocol for STK36 antibody 12559-1-AP | Download protocol |
| IP protocol for STK36 antibody 12559-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dev Biol Mammalian Fused is essential for sperm head shaping and periaxonemal structure formation during spermatogenesis. | ||
FASEB J The putative protein kinase Stk36 is essential for ciliogenesis and CSF flow by associating with Ulk4 | ||
Sci Rep Serine/threonine kinase 36 induced epithelial-mesenchymal transition promotes docetaxel resistance in prostate cancer |