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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated STIP1 Monoclonal antibody

STIP1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B5H2

Cat no : CL488-68155

Synonyms

HOP, Hsc70/Hsp90 organizing protein, IEF SSP 3521, P60, STI1, STI1L, STIP1

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-68155

    IF Staining of HeLa using CL488-68155

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 STIP1 antibody (CL488-68155, Clone: 1B5H2 ) at dilution of 1:100.

Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68155-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-68155 targets STIP1 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STIP1 fusion protein Ag7851 相同性解析による交差性が予測される生物種
Full Name stress-induced-phosphoprotein 1
Calculated molecular weight 63 kDa
Observed molecular weight61 kDa
GenBank accession numberBC002987
Gene symbol STIP1
Gene ID (NCBI) 10963
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STIP1, also named as Hop and NY-REN-11. Its expression is low in the hypothalamus. STIP1 has some isoforms with MW 62 kDa, 68 kDa and 59 kDa. Acts as a co-chaperone for HSP90AA1.Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90, Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. STIP1 interacts with PACRG, EEF1AKMT3, FLCN, FNIP1 and FNIP2 (PMID:24880080).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 STIP1 antibody CL488-68155Download protocol
Standard Protocols
Click here to view our Standard Protocols