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CoraLite® Plus 647-conjugated STIM1 Polyclonal antibody
STIM1 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
Cat no : CL647-11565
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL647-11565 targets STIM1 in FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | STIM1 fusion protein Ag2140 相同性解析による交差性が予測される生物種 |
| Full Name | stromal interaction molecule 1 |
| Calculated molecular weight | 685 aa, 77 kDa |
| Observed molecular weight | 77-90 kDa |
| GenBank accession number | BC021300 |
| Gene symbol | STIM1 |
| Gene ID (NCBI) | 6786 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms. Catalog#11565-1-AP is rabbit polyclonal antibody raised against the N-terminal of human STIM1.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 STIM1 antibody CL647-11565 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
