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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®555-conjugated STAT4 Monoclonal antibody

STAT4 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

4A8C9

Cat no : CL555-67568

Synonyms

SLEB11, STAT4

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL555-67568

    IF Staining of HepG2 using CL555-67568

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 STAT4 antibody (CL555-67568, Clone: 4A8C9 ) at dilution of 1:200.

  • FC experiment of mouse Th1 using CL555-67568

    FC experiment of mouse Th1 using CL555-67568

    1X10^6 mouse Th1 were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human STAT4 (CL555-67568, Clone:4A8C9) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy STAT4 Ready-To-Use IHC Kit. STAT4 primary antibody included.

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL555-67568 targets STAT4 in IF applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STAT4 fusion protein Ag19545 相同性解析による交差性が予測される生物種
Full Name signal transducer and activator of transcription 4
Calculated molecular weight 748 aa, 86 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC031212
Gene symbol STAT4
Gene ID (NCBI) 6775
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The JAK/STAT pathway is an extensive signaling pathway downstream of cytokine receptors. STATs are cytosolic proteins with a common structure consisting of an N-terminal oligomerization domain, which favors formation of STAT dimers, followed by a DNA-binding domain and a C-terminal SRC homology-2 (SH2) domain, which is involved in association between STATs and receptors[PMID:22383755]. Signal Transducer and Activator of Transcription 4 (STAT4) is a transcription factor that is activated by IL-12 signaling and promotes Th1-cell differentiation and IFN-γ production [PMID:21998209].

Protocols

Product Specific Protocols
IF protocol for CL555 STAT4 antibody CL555-67568Download protocol
Standard Protocols
Click here to view our Standard Protocols