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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STAP2 Polyclonal antibody (30516-1-AP)

    WB analysis using 30516-1-AP (same clone as 30516-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30516-1-AP (STAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30516-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using STAP2 Polyclonal antibody (30516-1-AP)

    WB analysis using 30516-1-AP (same clone as 30516-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30516-1-AP (STAP2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30516-1-PBS in a different storage buffer formulation.

STAP2 Polyclonal antibody, PBS Only

STAP2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 30516-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

BKS, Breast tumor kinase substrate, BRK substrate, Signal-transducing adaptor protein 2, STAP-2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30516-1-PBS targets STAP2 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag28818

Product name: Recombinant human STAP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 201-403 aa of BC000795

Sequence: VRHYKVKREGPKYVIDVEQPFSCTSLDAVVNYFVSHTKKALVPFLLDEDYEKVLGYVEADKENGENVWVAPSAPGPGPAPCTGGPKPLSPASSQDKLPPLPPLPNQEENYVTPIGDGPAVDYENQDVASSSWPVILKPKKLPKPPAKLPKPPVGPKPEPKVFNGGLGRKLPVSSAQPLFPTAGLADMTAELQKKLEKRRALEH

相同性解析による交差性が予測される生物種
Full Name signal transducing adaptor family member 2
Calculated molecular weight 45 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC000795
Gene Symbol STAP2
Gene ID (NCBI) 55620
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGK3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

STAP2, also known as BKS, belongs to the signal-transducing adaptor protein (STAP) family, The family includes STAP1 and STAP2, is a group of adaptor molecules involved in regulating multiple signaling pathways. The expression level of STAP2 in tubular cells is significantly higher than that in other renal cells, accounting for approximately 70%. In tubular epithelial cells, which are primarily affected by renal injury, STAP2 is mainly localized in the cytoplasm (PMID: 39533293).

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