Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, LNCaP cells, MCF-7 cells, MDA-MB-231 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30516-1-AP targets STAP2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28818 Product name: Recombinant human STAP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 201-403 aa of BC000795 Sequence: VRHYKVKREGPKYVIDVEQPFSCTSLDAVVNYFVSHTKKALVPFLLDEDYEKVLGYVEADKENGENVWVAPSAPGPGPAPCTGGPKPLSPASSQDKLPPLPPLPNQEENYVTPIGDGPAVDYENQDVASSSWPVILKPKKLPKPPAKLPKPPVGPKPEPKVFNGGLGRKLPVSSAQPLFPTAGLADMTAELQKKLEKRRALEH 相同性解析による交差性が予測される生物種 |
| Full Name | signal transducing adaptor family member 2 |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC000795 |
| Gene Symbol | STAP2 |
| Gene ID (NCBI) | 55620 |
| RRID | AB_3086344 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UGK3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
STAP2, also known as BKS, belongs to the signal-transducing adaptor protein (STAP) family, The family includes STAP1 and STAP2, is a group of adaptor molecules involved in regulating multiple signaling pathways. The expression level of STAP2 in tubular cells is significantly higher than that in other renal cells, accounting for approximately 70%. In tubular epithelial cells, which are primarily affected by renal injury, STAP2 is mainly localized in the cytoplasm (PMID: 39533293).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for STAP2 antibody 30516-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |