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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STAP1 Polyclonal antibody (31732-1-AP)

    WB analysis using 31732-1-AP (same clone as 31732-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31732-1-AP (STAP1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31732-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using STAP1 Polyclonal antibody (31732-1-AP)

    IF Staining of A431 using 31732-1-AP (same clone as 31732-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using STAP1 antibody (31732-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31732-1-PBS in a different storage buffer formulation.

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STAP1 Polyclonal antibody, PBS Only

STAP1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 31732-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

STAP-1, Docking protein BRDG1, BRDG1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31732-1-PBS targets STAP1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STAP1 fusion protein Ag35196 相同性解析による交差性が予測される生物種
Full Name signal transducing adaptor family member 1
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC014958
Gene Symbol STAP1
Gene ID (NCBI) 26228
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9ULZ2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Signal transducing adaptor protein 1 (STAP1), also known as BRDG1, contains a proline-rich region, a pleckstrin homology (PH) domain, and a region in the carboxy terminal half with similarity to the Src Homology 2 (SH2) domain. STAP1 acts downstream of the Tec tyrosine kinase and participates in a positive feedback loop by increasing the activity of Tec (PMID: 10518561).