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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STAP1 Polyclonal antibody (31732-1-AP)

    WB analysis using 31732-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31732-1-AP (STAP1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using STAP1 Polyclonal antibody (31732-1-AP)

    IF Staining of A431 using 31732-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using STAP1 antibody (31732-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

STAP1 Polyclonal antibody

STAP1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31732-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

STAP-1, Docking protein BRDG1, BRDG1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRaji cells, Ramos cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31732-1-AP targets STAP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STAP1 fusion protein Ag35196 相同性解析による交差性が予測される生物種
Full Name signal transducing adaptor family member 1
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC014958
Gene Symbol STAP1
Gene ID (NCBI) 26228
RRIDAB_3670102
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9ULZ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Signal transducing adaptor protein 1 (STAP1), also known as BRDG1, contains a proline-rich region, a pleckstrin homology (PH) domain, and a region in the carboxy terminal half with similarity to the Src Homology 2 (SH2) domain. STAP1 acts downstream of the Tec tyrosine kinase and participates in a positive feedback loop by increasing the activity of Tec (PMID: 10518561).

Protocols

Product Specific Protocols
WB protocol for STAP1 antibody 31732-1-APDownload protocol
IF protocol for STAP1 antibody 31732-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols