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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ST3GAL6 Polyclonal antibody (13154-1-AP)

    WB analysis using 13154-1-AP (same clone as 13154-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13154-1-AP (ST3GAL6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13154-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using ST3GAL6 Polyclonal antibody (13154-1-AP)

    WB analysis of human heart using 13154-1-AP (same clone as 13154-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 13154-1-AP (ST3GAL6 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13154-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ST3GAL6 Polyclonal antibody (13154-1-AP)

    IHC staining of human liver using 13154-1-AP (same clone as 13154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13154-1-AP (ST3GAL6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13154-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using ST3GAL6 Polyclonal antibody (13154-1-AP)

    IHC staining of human urothelial carcinoma using 13154-1-AP (same clone as 13154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 13154-1-AP (ST3GAL6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13154-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using ST3GAL6 Polyclonal antibody (13154-1-AP)

    IHC staining of human urothelial carcinoma using 13154-1-AP (same clone as 13154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 13154-1-AP (ST3GAL6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13154-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ST3GAL6 Polyclonal antibody (13154-1-AP)

    IHC staining of human liver using 13154-1-AP (same clone as 13154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13154-1-AP (ST3GAL6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13154-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ST3GAL6 Polyclonal antibody, PBS Only

ST3GAL6 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13154-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13154-1-PBS targets ST3GAL6 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3782

Product name: Recombinant human ST3GAL6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 25-331 aa of BC023312

Sequence: GTNVYWVAPVEMKRRNKIQPCLSKPAFASLLRFHQFHPFLCAADFRKIASLYGSDKFDLPYGMRTSAEYFRLALSKLQSCDLFDEFDNIPCKKCVVVGNGGVLKNKTLGEKIDSYDVIIRMNNGPVLGHEEEVGRRTTFRLFYPESVFSDPIHNDPNTTVILTAFKPHDLRWLLELLMGDKINTNGFWKKPALNLIYKPYQIRILDPFIIRTAAYELLHFPKVFPKNQKPKHPTTGIIAITLAFYICHEVHLAGFKYNFSDLKSPLHYYGNATMSLMNKNAYHNVTAEQLFLKDIIEKNLVINLTQD

相同性解析による交差性が予測される生物種
Full Name ST3 beta-galactoside alpha-2,3-sialyltransferase 6
Calculated molecular weight 331 aa, 38 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC023312
Gene Symbol ST3GAL6
Gene ID (NCBI) 10402
RRIDAB_10646441
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y274
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ST3GAL6, also named as SIAT10, belongs to the glycosyltransferase 29 family. ST3GAL6 is involved in the synthesis of sialyl-paragloboside, which is a precursor of sialyl-Lewis X determinant. ST3GAL6 has a alpha-2,3-sialyltransferase activity toward Gal-beta1,4-GlcNAc structure on glycoproteins and glycolipids. ST3GAL6 is known to promote cell adhesion and migration of multiple myeloma cells by increasing the synthesis of selectin ligands. ST3GAL6 has 2 isoforms with the molecular mass of 25 and 38 kDa. Sometimes higher molecular weight about 50-60 kDa can also be observed, which may be a glycosylated modified variant of ST3GAL6. (PMID: 31914669, 30220088)

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