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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using SRP68 Polyclonal antibody (11585-1-AP)

    WB analysis of mouse brain using 11585-1-AP (same clone as 11585-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11585-1-AP (SRP68 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using SRP68 Polyclonal antibody (11585-1-AP)

    WB analysis of human brain using 11585-1-AP (same clone as 11585-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11585-1-AP (SRP68 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using SRP68 Polyclonal antibody (11585-1-AP)

    IP experiment of mouse kidney using 11585-1-AP (same clone as 11585-1-PBS)

    IP result of anti-SRP68 (IP:11585-1-AP, 4ug; Detection:11585-1-AP 1:400) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SRP68 Polyclonal antibody (11585-1-AP)

    IHC staining of human gliomas using 11585-1-AP (same clone as 11585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11585-1-AP (SRP68 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using SRP68 Polyclonal antibody (11585-1-AP)

    IHC staining of rat testis using 11585-1-AP (same clone as 11585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11585-1-AP (SRP68 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using SRP68 Polyclonal antibody (11585-1-AP)

    IHC staining of rat testis using 11585-1-AP (same clone as 11585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11585-1-AP (SRP68 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SRP68 Polyclonal antibody (11585-1-AP)

    IHC staining of human gliomas using 11585-1-AP (same clone as 11585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11585-1-AP (SRP68 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SRP68 Polyclonal antibody (11585-1-AP)

    IF Staining of HepG2 using 11585-1-AP (same clone as 11585-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SRP68 antibody (11585-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SRP68 Polyclonal antibody, PBS Only

SRP68 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11585-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

SRP 68, Signal recognition particle subunit SRP68

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11585-1-PBS targets SRP68 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2177

Product name: Recombinant human SRP68 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 262-596 aa of BC020238

Sequence: MSEVEWRGRTVPVKIDKVRIFLLGLADNEAAIVQAESEETKERLFESMLSECRDAIQVVREELKPDQKQRDYILEGEPGKVSNLQYLHSYLTYIKLSTAIKRNENMAKGLQRALLQQQPEDDSKRSPRPQDLIRLYDIILQNLVELLQLPGLEEDKAFQKEIGLKTLVFKAYRCFFIAQSYVLVKKWSEALVLYDRVLKYANEVNSDAGAFKNSLKDLPDVQELITQVRSEKCSLQAAAILDANDAHQTETSSSQVKDNKPLVERFETFCLDPSLVTKQANLVHFPPGFQPIPCKPLFFDLALNHVAFPPLEDKLEQKTKSGLTGYIKGIFGFRS

相同性解析による交差性が予測される生物種
Full Name signal recognition particle 68kDa
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC020238
Gene Symbol SRP68
Gene ID (NCBI) 6730
RRIDAB_2286586
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHB9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of secretory proteins to the rough endoplasmic reticulum (ER) membrane. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function. SRP68 is one component of the ribonucleprotein complex, and forms a heterodimer with SRP72.

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