Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, human brain tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human gliomas tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
Product Information
11585-1-AP targets SRP68 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SRP68 fusion protein Ag2177 相同性解析による交差性が予測される生物種 |
| Full Name | signal recognition particle 68kDa |
| Calculated molecular weight | 68 kDa |
| Observed molecular weight | 68 kDa |
| GenBank accession number | BC020238 |
| Gene Symbol | SRP68 |
| Gene ID (NCBI) | 6730 |
| RRID | AB_2286586 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UHB9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of secretory proteins to the rough endoplasmic reticulum (ER) membrane. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function. SRP68 is one component of the ribonucleprotein complex, and forms a heterodimer with SRP72.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SRP68 antibody 11585-1-AP | Download protocol |
| IHC protocol for SRP68 antibody 11585-1-AP | Download protocol |
| IF protocol for SRP68 antibody 11585-1-AP | Download protocol |
| IP protocol for SRP68 antibody 11585-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) Targeting N4-acetylcytidine suppresses hepatocellular carcinoma progression by repressing eEF2-mediated HMGB2 mRNA translation
| ||
Nat Commun The Perlman syndrome DIS3L2 exoribonuclease safeguards endoplasmic reticulum-targeted mRNA translation and calcium ion homeostasis. | ||
Nucleic Acids Res The pioneer round of translation ensures proper targeting of ER and mitochondrial proteins. | ||
Nucleic Acids Res Implication of the SMN complex in the biogenesis and steady state level of the signal recognition particle. | ||
Nucleic Acids Res Protein Hit1, a novel box C/D snoRNP assembly factor, controls cellular concentration of the scaffolding protein Rsa1 by direct interaction. | ||