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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPTLC2 Monoclonal antibody (68094-1-Ig)

    WB analysis using 68094-1-Ig (same clone as 68094-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68094-1-Ig (SPTLC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68094-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SPTLC2 Monoclonal antibody (68094-1-Ig)

    WB analysis of HEK-293 using 68094-1-Ig (same clone as 68094-1-PBS)

    WB result of SPTLC2 antibody (68094-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPTLC2 transfected HEK-293 cells. This data was developed using the same antibody clone with 68094-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SPTLC2 Monoclonal antibody, PBS Only

SPTLC2 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68094-1-PBS
Clone No.2G6A12

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

KIAA0526, LCB 2, LCB2, LCB2a, Serine palmitoyltransferase 2, SPT 2, SPT2, SPTLC2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68094-1-PBS targets SPTLC2 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28380

Product name: Recombinant human SPTLC2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 254-562 aa of BC005123

Sequence: CLILSDELNHASLVLGARLSGATIRIFKHNNMQSLEKLLKDAIVYGQPRTRRPWKKILILVEGIYSMEGSIVRLPEVIALKKKYKAYLYLDEAHSIGALGPTGRGVVEYFGLDPEDVDVMMGTFTKSFGASGGYIGGKKELIDYLRTHSHSAVYATSLSPPVVEQIITSMKCIMGQDGTSLGKECVQQLAENTRYFRRRLKEMGFIIYGNEDSPVVPLMLYMPAKIGAFGREMLKRNIGVVVVGFPATPIIESRARFCLSAAHTKEILDTALKEIDEVGDLLQLKYSRHRLVPLLDRPFDETTYEETED

相同性解析による交差性が予測される生物種
Full Name serine palmitoyltransferase, long chain base subunit 2
Calculated molecular weight 63 kDa
Observed molecular weight60 kDa
GenBank accession numberBC005123
Gene Symbol SPTLC2
Gene ID (NCBI) 9517
RRIDAB_2918831
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO15270
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Serine palmitoyltransferase long chain base subunit 2 (SPTLC2) is one of the subunits of the heterodimeric molecule Serine palmitoyltransferase (SPT). Sptlc2 deficiency not only affected T cell sphingolipid anabolism but also led to prolonged activation of the mechanistic target of rapamycin complex 1 (PMID: 30952607).

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