Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, HepG2 cells, A549 cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68094-1-Ig targets SPTLC2 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28380 Product name: Recombinant human SPTLC2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 254-562 aa of BC005123 Sequence: CLILSDELNHASLVLGARLSGATIRIFKHNNMQSLEKLLKDAIVYGQPRTRRPWKKILILVEGIYSMEGSIVRLPEVIALKKKYKAYLYLDEAHSIGALGPTGRGVVEYFGLDPEDVDVMMGTFTKSFGASGGYIGGKKELIDYLRTHSHSAVYATSLSPPVVEQIITSMKCIMGQDGTSLGKECVQQLAENTRYFRRRLKEMGFIIYGNEDSPVVPLMLYMPAKIGAFGREMLKRNIGVVVVGFPATPIIESRARFCLSAAHTKEILDTALKEIDEVGDLLQLKYSRHRLVPLLDRPFDETTYEETED 相同性解析による交差性が予測される生物種 |
| Full Name | serine palmitoyltransferase, long chain base subunit 2 |
| Calculated molecular weight | 63 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC005123 |
| Gene Symbol | SPTLC2 |
| Gene ID (NCBI) | 9517 |
| RRID | AB_2918831 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O15270 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Serine palmitoyltransferase long chain base subunit 2 (SPTLC2) is one of the subunits of the heterodimeric molecule Serine palmitoyltransferase (SPT). Sptlc2 deficiency not only affected T cell sphingolipid anabolism but also led to prolonged activation of the mechanistic target of rapamycin complex 1 (PMID: 30952607).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SPTLC2 antibody 68094-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |