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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated SPTLC1 Monoclonal antibody

SPTLC1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D11D7

Cat no : CL594-66899

Synonyms

HSAN, HSAN1, HSN1, LBC1, LCB 1, LCB1, Serine palmitoyltransferase 1, SPT 1, SPT1, SPTI, SPTLC1

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-66899

    IF Staining of HepG2 using CL594-66899

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 SPTLC1 antibody (CL594-66899, Clone: 2D11D7 ) at dilution of 1:100.

Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66899-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66899 targets SPTLC1 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SPTLC1 fusion protein Ag7876 相同性解析による交差性が予測される生物種
Full Name serine palmitoyltransferase, long chain base subunit 1
Calculated molecular weight 53 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC007085
Gene symbol SPTLC1
Gene ID (NCBI) 10558
RRIDAB_2920053
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

SPTLC1 is a subunit of serine palmitoyltransferase (SPT) which is the key enzyme in sphingolipid biosynthesis and is essential for embryogenesis and cell survival. Mutations in the SPTLC1 gene (C133W, C133Y, V144D, and G387A) were reported to be responsible for the development of an inherited sensory neuropathy (hereditary sensory neuropathy type I, HSN1).

Protocols

Product Specific Protocols
IF protocol for CL594 SPTLC1 antibody CL594-66899Download protocol
Standard Protocols
Click here to view our Standard Protocols