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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPTB Polyclonal antibody (26936-1-AP)

    WB analysis using 26936-1-AP (same clone as 26936-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26936-1-AP (SPTB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26936-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using SPTB Polyclonal antibody (26936-1-AP)

    IF Staining of U2OS using 26936-1-AP (same clone as 26936-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using SPTB antibody (26936-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26936-1-PBS in a different storage buffer formulation.

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SPTB Polyclonal antibody, PBS Only

SPTB Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 26936-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Spectrin beta chain, erythrocytic, Beta-I spectrin, Beta I spectrin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26936-1-PBS targets SPTB in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPTB fusion protein Ag25614 相同性解析による交差性が予測される生物種
Full Name spectrin, beta, erythrocytic
Observed molecular weight 246 kDa
GenBank accession numberBC136284
Gene Symbol SPTB
Gene ID (NCBI) 6710
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11277
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Beta I spectrin also known as SPTB, SPTB1, belongs to the spectrin family. Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Mutations in SPTB have been associated with Elliptocytosis 3, spherocytosis type 2, and neonatal hemolytic anemia(PMID: 8018926, 8102379, 9005995).