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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPRY1 Polyclonal antibody (17813-1-AP)

    WB analysis using 17813-1-AP (same clone as 17813-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17813-1-AP (SPRY1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17813-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SPRY1 Polyclonal antibody (17813-1-AP)

    WB analysis using 17813-1-AP (same clone as 17813-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17813-1-AP (SPRY1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17813-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SPRY1 Polyclonal antibody (17813-1-AP)

    WB analysis using 17813-1-AP (same clone as 17813-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17813-1-AP (SPRY1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17813-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A2780 cells using SPRY1 Polyclonal antibody (17813-1-AP)

    IP experiment of A2780 using 17813-1-AP (same clone as 17813-1-PBS)

    IP result of anti-SPRY1 (IP:17813-1-AP, 4ug; Detection:17813-1-AP 1:3000) with A2780 cells lysate 1000 ug. This data was developed using the same antibody clone with 17813-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HT-29 cells using SPRY1 Polyclonal antibody (17813-1-AP)

    IP experiment of HT-29 using 17813-1-AP (same clone as 17813-1-PBS)

    IP result of anti-SPRY1 (IP:17813-1-AP, 4ug; Detection:17813-1-AP 1:3000) with HT-29 cells lysate 1760 ug. This data was developed using the same antibody clone with 17813-1-PBS in a different storage buffer formulation.

SPRY1 Polyclonal antibody, PBS Only

SPRY1 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 17813-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

hSPRY1, Protein sprouty homolog 1, Spry 1, Spry-1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17813-1-PBS targets SPRY1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12129

Product name: Recombinant human SPRY1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-319 aa of BC063856

Sequence: MDPQNQHGSGSSLVVIQQPSLDSRQRLDYEREIQPTAILSLDQIKAIRGSNEYTEGPSVVKRPAPRTAPRQEKHERTHEIIPINVNNNYEHRHTSHLGHAVLPSNARGPILSRSTSTGSAASSGSNSSASSEQGLLGRSPPTRPVPGHRSERAIRTQPKQLIVDDLKGSLKEDLTQHKFICEQCGKCKCGECTAPRTLPSCLACNRQCLCSAESMVEYGTCMCLVKGIFYHCSNDDEGDSYSDNPCSCSQSHCCSRYLCMGAMSLFLPCLLCYPPAKGCLKLCRRCYDWIHRPGCRCKNSNTVYCKLESCPSRGQGKPS

相同性解析による交差性が予測される生物種
Full Name sprouty homolog 1, antagonist of FGF signaling (Drosophila)
Calculated molecular weight 319 aa, 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC063856
Gene Symbol SPRY1
Gene ID (NCBI) 10252
RRIDAB_3741933
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43609
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Sprouty homolog 1 (SPRY1) belongs to the sprouty family. Spry-1 is a negative regulator of receptor tyrosine kinase signalling (PMID: 36543535). Spry1 and its related proteins have also been implicated as negative regulators of RhoA and its downstream effector Rho kinase through the non-canonical Wnt signaling (PMID: 19671746, PMID: 15184877). Sprouty-1 and Sprouty-2 proteins are expressed in early embryonic lung both in the epithelial cells of distal tubules and in peripheral mesenchymal cells (PMID: 22236546). Spry1 is a cytoplasmic protein that plays a critical role in kidney development and is primarily known to inhibit the Ras/MEK/ERK pathway (PMID: 21310958).

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