• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SPRY1 Polyclonal antibody (17813-1-AP)

    WB analysis using 17813-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17813-1-AP (SPRY1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SPRY1 Polyclonal antibody (17813-1-AP)

    WB analysis using 17813-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17813-1-AP (SPRY1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A2780 cells using SPRY1 Polyclonal antibody (17813-1-AP)

    IP experiment of A2780 using 17813-1-AP

    IP result of anti-SPRY1 (IP:17813-1-AP, 4ug; Detection:17813-1-AP 1:3000) with A2780 cells lysate 1000 ug.

  • Immunoprecipitation (IP) experiment of HT-29 cells using SPRY1 Polyclonal antibody (17813-1-AP)

    IP experiment of HT-29 using 17813-1-AP

    IP result of anti-SPRY1 (IP:17813-1-AP, 4ug; Detection:17813-1-AP 1:3000) with HT-29 cells lysate 1760 ug.

SPRY1 Polyclonal antibody

SPRY1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 17813-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

hSPRY1, Protein sprouty homolog 1, Spry 1, Spry-1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA2780 cells, HEK-293 cells, HepG2 cells
Positive IP detected inHT-29 cells, A2780 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17813-1-AP targets SPRY1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12129

Product name: Recombinant human SPRY1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-319 aa of BC063856

Sequence: MDPQNQHGSGSSLVVIQQPSLDSRQRLDYEREIQPTAILSLDQIKAIRGSNEYTEGPSVVKRPAPRTAPRQEKHERTHEIIPINVNNNYEHRHTSHLGHAVLPSNARGPILSRSTSTGSAASSGSNSSASSEQGLLGRSPPTRPVPGHRSERAIRTQPKQLIVDDLKGSLKEDLTQHKFICEQCGKCKCGECTAPRTLPSCLACNRQCLCSAESMVEYGTCMCLVKGIFYHCSNDDEGDSYSDNPCSCSQSHCCSRYLCMGAMSLFLPCLLCYPPAKGCLKLCRRCYDWIHRPGCRCKNSNTVYCKLESCPSRGQGKPS

相同性解析による交差性が予測される生物種
Full Name sprouty homolog 1, antagonist of FGF signaling (Drosophila)
Calculated molecular weight 319 aa, 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC063856
Gene Symbol SPRY1
Gene ID (NCBI) 10252
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43609
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sprouty homolog 1 (SPRY1) belongs to the sprouty family. Spry-1 is a negative regulator of receptor tyrosine kinase signalling (PMID: 36543535). Spry1 and its related proteins have also been implicated as negative regulators of RhoA and its downstream effector Rho kinase through the non-canonical Wnt signaling (PMID: 19671746, PMID: 15184877). Sprouty-1 and Sprouty-2 proteins are expressed in early embryonic lung both in the epithelial cells of distal tubules and in peripheral mesenchymal cells (PMID: 22236546). Spry1 is a cytoplasmic protein that plays a critical role in kidney development and is primarily known to inhibit the Ras/MEK/ERK pathway (PMID: 21310958).

Protocols

Product Specific Protocols
IP protocol for SPRY1 antibody 17813-1-APDownload protocol
WB protocol for SPRY1 antibody 17813-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...