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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPIRE1 Polyclonal antibody (11295-1-AP)

    WB analysis using 11295-1-AP (same clone as 11295-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11295-1-AP (SPIRE1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SPIRE1 Polyclonal antibody (11295-1-AP)

    IHC staining of human lung cancer using 11295-1-AP (same clone as 11295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11295-1-AP (SPIRE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 11295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SPIRE1 Polyclonal antibody (11295-1-AP)

    IHC staining of human lung cancer using 11295-1-AP (same clone as 11295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11295-1-AP (SPIRE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 11295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using SPIRE1 Polyclonal antibody (11295-1-AP)

    IHC staining of mouse stomach using 11295-1-AP (same clone as 11295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 11295-1-AP (SPIRE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11295-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SPIRE1 Polyclonal antibody (11295-1-AP)

    IF Staining of mouse brain using 11295-1-AP (same clone as 11295-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SPIRE1 antibody (11295-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11295-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SPIRE1 Polyclonal antibody (11295-1-AP)

    IF Staining of mouse brain using 11295-1-AP (same clone as 11295-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SPIRE1 antibody (11295-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11295-1-PBS in a different storage buffer formulation.

SPIRE1 Polyclonal antibody, PBS Only

SPIRE1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 11295-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

KIAA1135, Protein spire homolog 1, Spir 1, SPIR1, Spir-1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11295-1-PBS targets SPIRE1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1798

Product name: Recombinant human SPIRE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 600-756 aa of BC016825

Sequence: GRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESSMRSEKPSTAHHRPLRSIARFSSKSKSMDKSDEELQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSPGPSEYCPSERTISEI

相同性解析による交差性が予測される生物種
Full Name spire homolog 1 (Drosophila)
Calculated molecular weight 64 kDa
Observed molecular weight50-60 kDa, 95 kDa
GenBank accession numberBC016825
Gene Symbol SPIRE1
Gene ID (NCBI) 56907
RRIDAB_3085370
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08AE8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SPIRE1, Protein spire homolog 1, belongs to the family of Wiskott-Aldrich homology region-2 (WH2) proteins (PMID: 11747823). SPIRE1 acts as an actin nucleation factor, associated with the slow-growing pointed end of the new filament (PMID: 11747823, PMID: 21620703). SPIRE1 and FMN2 promote assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (PMID: 26287480).

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