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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SPIRE1 Polyclonal antibody

SPIRE1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 11295-1-AP

Synonyms

KIAA1135, Protein spire homolog 1, Spir 1, SPIR1, spire homolog 1 (Drosophila), SPIRE1

Validation Data Gallery

Applications:
  • WB analysis using 11295-1-AP

    WB analysis using 11295-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11295-1-AP (SPIRE1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 11295-1-AP

    IHC staining of human lung cancer using 11295-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11295-1-AP (SPIRE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with .

  • IHC staining of human lung cancer using 11295-1-AP

    IHC staining of human lung cancer using 11295-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11295-1-AP (SPIRE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with .

Tested Applications

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11295-1-AP targets SPIRE1 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPIRE1 fusion protein Ag1798 相同性解析による交差性が予測される生物種
Full Name spire homolog 1 (Drosophila)
Calculated molecular weight 64 kDa
Observed molecular weight50-60 kDa, 95 kDa
GenBank accession numberBC016825
Gene symbol SPIRE1
Gene ID (NCBI) 56907
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPIRE1, Protein spire homolog 1, belongs to the family of Wiskott-Aldrich homology region-2 (WH2) proteins (PMID: 11747823). SPIRE1 acts as an actin nucleation factor, associated with the slow-growing pointed end of the new filament (PMID: 11747823, PMID: 21620703). SPIRE1 and FMN2 promote assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (PMID: 26287480).

Protocols

Product Specific Protocols
WB protocol for SPIRE1 antibody 11295-1-APDownload protocol
IHC protocol for SPIRE1 antibody 11295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols