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Validation Data Gallery

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  • Western Blot (WB) analysis of Daudi cells using SPI1 Monoclonal antibody (66618-2-Ig)

    WB analysis of Daudi using 66618-2-Ig (same clone as 66618-2-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using SPI1 Monoclonal antibody (66618-2-Ig)

    WB analysis of RAW 264.7 using 66618-2-Ig (same clone as 66618-2-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat spleen tissue using SPI1 Monoclonal antibody (66618-2-Ig)

    WB analysis of rat spleen using 66618-2-Ig (same clone as 66618-2-PBS)

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using SPI1 Monoclonal antibody (66618-2-Ig)

    WB analysis of Raji using 66618-2-Ig (same clone as 66618-2-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using SPI1 Monoclonal antibody (66618-2-Ig)

    IHC staining of mouse spleen using 66618-2-Ig (same clone as 66618-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Human tonsillitis tissue using SPI1 Monoclonal antibody (66618-2-Ig)

    IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)

    Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:4000 (under 20x lens). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Human tonsillitis tissue using SPI1 Monoclonal antibody (66618-2-Ig)

    IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)

    Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:4000 (under 20x lens). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Human tonsillitis tissue using SPI1 Monoclonal antibody (66618-2-Ig)

    IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)

    Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:2000 (under 20x lens). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using SPI1 Monoclonal antibody (66618-2-Ig)

    IHC staining of mouse spleen using 66618-2-Ig (same clone as 66618-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

SPI1 Monoclonal antibody, PBS Only

SPI1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66618-2-PBS
Clone No.2H3D3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

2H3D3, 31 kDa transforming protein, 31 kDa-transforming protein, OF, PU.1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66618-2-PBS targets SPI1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name spleen focus forming virus (SFFV) proviral integration oncogene spi1
Calculated molecular weight 31 kDa
Observed molecular weight 32-38 kDa
GenBank accession numberNM_003120
Gene Symbol SPI1
Gene ID (NCBI) 6688
ENSEMBL Gene IDENSG00000066336
RRIDAB_2881978
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP17947
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SPI1, also named as 31 kDa-transforming protein, is a 270 amino acid protein, which belongs to the ETS family. SPI1 is highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the SPI1 gene due to the insertion of SFFV.

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