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SPI1 Monoclonal antibody

SPI1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

2H3D3

Cat no : 66618-2-Ig

Synonyms

31 kDa transforming protein, OF, PU.1, SFPI1, SPI 1, SPI A, SPI1, Transcription factor PU.1

Validation Data Gallery

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  • Western Blot (WB) analysis of Daudi cells using SPI1 Monoclonal antibody (66618-2-Ig)

    WB analysis of Daudi using 66618-2-Ig

    Daudi cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using SPI1 Monoclonal antibody (66618-2-Ig)

    WB analysis of RAW 264.7 using 66618-2-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat spleen tissue using SPI1 Monoclonal antibody (66618-2-Ig)

    WB analysis of rat spleen using 66618-2-Ig

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using SPI1 Monoclonal antibody (66618-2-Ig)

    WB analysis of Raji using 66618-2-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using SPI1 Monoclonal antibody (66618-2-Ig)

    IHC staining of mouse spleen using 66618-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using SPI1 Monoclonal antibody (66618-2-Ig)

    IHC staining of mouse spleen using 66618-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inDaudi cells, Raji cells, RAW 264.7 cells, rat spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66618-2-Ig targets SPI1 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name spleen focus forming virus (SFFV) proviral integration oncogene spi1
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 32-38 kDa
GenBank Accession NumberNM_003120
Gene Symbol SPI1
Gene ID (NCBI) 6688
RRIDAB_2881978
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPI1, also named as 31 kDa-transforming protein, is a 270 amino acid protein, which belongs to the ETS family. SPI1 is highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the SPI1 gene due to the insertion of SFFV.

Protocols

Product Specific Protocols
WB protocol for SPI1 antibody 66618-2-IgDownload protocol
IHC protocol for SPI1 antibody 66618-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,WB

Discov Oncol

SPI1 is a prognostic biomarker of immune infiltration and immunotherapy efficacy in clear cell renal cell carcinoma

Authors - Huayi Feng
View Article
humanWB

Cancers (Basel)

ACAP1 Deficiency Predicts Inferior Immunotherapy Response in Solid Tumors

Authors - Qiyi Yi
View Article
humanWB,IF,CoIP

Exp Cell Res

IRF1 suppresses colon cancer proliferation by reducing SPI1-mediated transcriptional activation of GPX4 and promoting ferroptosis

Authors - Yilin Chen
View Article