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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPATA5 Polyclonal antibody (32104-1-AP)

    WB analysis using 32104-1-AP (same clone as 32104-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32104-1-AP (SPATA5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32104-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SPATA5 Polyclonal antibody (32104-1-AP)

    IHC staining of human liver using 32104-1-AP (same clone as 32104-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 32104-1-AP (SPATA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32104-1-PBS in a different storage buffer formulation.

SPATA5 Polyclonal antibody, PBS Only

SPATA5 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 32104-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

AFG2 AAA ATPase homolog A, AFG2A, ATPase family gene 2 protein homolog A, EC:3.6.4.10, Ribosome biogenesis protein SPATA5

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

32104-1-PBS targets SPATA5 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag36838

Product name: Recombinant human SPATA5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 100-300 aa of BC048217

Sequence: WPMAGFPGGKVGLSEMAQKNVGVRPGDAIQVQPLVGAVLQAEEMDVALSDKDMEINEEELTGCILRKLDGKIVLPGNFLYCTFYGRPYKLQVLRVKGADGMILGGPQSDSDTDAQRMAFEQSSMETSSLELSLQLSQLDLEDTQIPTSRSTPYKPIDDRITNKASDVLLDVTQSPGDGSGLMLEEVTGLKCNFESAREGNE

相同性解析による交差性が予測される生物種
Full Name spermatogenesis associated 5
Calculated molecular weight97, 86, 75 kDa
Observed molecular weight100 kDa
GenBank accession numberBC048217
Gene Symbol SPATA5
Gene ID (NCBI) 166378
RRIDAB_3670193
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8NB90
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AAA+ ATPases SPATA5 is an ATP-dependent chaperone part of the 55LCC heterohexameric ATPase complex which is chromatin-associated and promotes replisome proteostasis to maintain replication fork progression and genome stability (PubMed:38554706). SPATA5 plays an essential role in the cytoplasmic maturation steps of pre-60S ribosomal particles by promoting the release of shuttling protein RSL24D1/RLP24 from the pre-ribosomal particles (PubMed:35354024, PubMed:38554706).

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