Validation Data Gallery
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Tested Applications
| Positive WB detected in | RT-4 cells, HEK-293T cells, U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32104-1-AP targets SPATA5 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SPATA5 fusion protein Ag36838 相同性解析による交差性が予測される生物種 |
| Full Name | spermatogenesis associated 5 |
| Calculated molecular weight | 97, 86, 75 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC048217 |
| Gene Symbol | SPATA5 |
| Gene ID (NCBI) | 166378 |
| RRID | AB_3670193 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8NB90 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AAA+ ATPases SPATA5 is an ATP-dependent chaperone part of the 55LCC heterohexameric ATPase complex which is chromatin-associated and promotes replisome proteostasis to maintain replication fork progression and genome stability (PubMed:38554706). SPATA5 plays an essential role in the cytoplasmic maturation steps of pre-60S ribosomal particles by promoting the release of shuttling protein RSL24D1/RLP24 from the pre-ribosomal particles (PubMed:35354024, PubMed:38554706).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SPATA5 antibody 32104-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |