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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPATA2 Polyclonal antibody (29321-1-AP)

    WB analysis using 29321-1-AP (same clone as 29321-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29321-1-AP (SPATA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29321-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using SPATA2 Polyclonal antibody (29321-1-AP)

    IHC staining of mouse lung using 29321-1-AP (same clone as 29321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29321-1-AP (SPATA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29321-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using SPATA2 Polyclonal antibody (29321-1-AP)

    IHC staining of mouse lung using 29321-1-AP (same clone as 29321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29321-1-AP (SPATA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29321-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SPATA2 Polyclonal antibody (29321-1-AP)

    IF Staining of HeLa using 29321-1-AP (same clone as 29321-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPATA2 antibody (29321-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29321-1-PBS in a different storage buffer formulation.

SPATA2 Polyclonal antibody, PBS Only

SPATA2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29321-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

KIAA0757, PD1, spermatogenesis associated 2, Spermatogenesis-associated protein 2, tamo

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29321-1-PBS targets SPATA2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30773

Product name: Recombinant human SPATA2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 330-520 aa of BC009481

Sequence: STQDDVDLYTDSEPRATYRRQDALRPDVWLLRNDAHSLYHKRSPPAKESALSKCQSCGLSCSSSLCQRCDSLLTCPPASKPSAFPSKASTHDSLAHGASLREKYPGQTQGLDRLPHLHSKSKPSTTPTSRCGFCNRPGATNTCTQCSKVSCDACLSAYHYDPCYKKSELHKFMPNNQLNYKSTQLSHLVYR

相同性解析による交差性が予測される生物種
Full Name spermatogenesis associated 2
Calculated molecular weight 58 kDa
Observed molecular weight58-60 kDa
GenBank accession numberBC009481
Gene Symbol SPATA2
Gene ID (NCBI) 9825
RRIDAB_2918282
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UM82
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The protein SPATA2 (spermatogenesis-associated protein 2) was initially shown to be highly expressed in testicular Sertoli cells, and these early reports suggested SPATA2 to play a role in spermatogenesis. More recently, it was discussed to be involved in the predisposition for psoriasis. SPATA2 is required for TNF-induced cell death. SPATA2 has a potential role during pancreatic development and β-cell proliferation.(PMID: 20119533/PMID: 27458237)

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