Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IHC detected in | mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29321-1-AP targets SPATA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30773 Product name: Recombinant human SPATA2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 330-520 aa of BC009481 Sequence: STQDDVDLYTDSEPRATYRRQDALRPDVWLLRNDAHSLYHKRSPPAKESALSKCQSCGLSCSSSLCQRCDSLLTCPPASKPSAFPSKASTHDSLAHGASLREKYPGQTQGLDRLPHLHSKSKPSTTPTSRCGFCNRPGATNTCTQCSKVSCDACLSAYHYDPCYKKSELHKFMPNNQLNYKSTQLSHLVYR 相同性解析による交差性が予測される生物種 |
| Full Name | spermatogenesis associated 2 |
| Calculated molecular weight | 58 kDa |
| Observed molecular weight | 58-60 kDa |
| GenBank accession number | BC009481 |
| Gene Symbol | SPATA2 |
| Gene ID (NCBI) | 9825 |
| RRID | AB_2918282 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UM82 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
The protein SPATA2 (spermatogenesis-associated protein 2) was initially shown to be highly expressed in testicular Sertoli cells, and these early reports suggested SPATA2 to play a role in spermatogenesis. More recently, it was discussed to be involved in the predisposition for psoriasis. SPATA2 is required for TNF-induced cell death. SPATA2 has a potential role during pancreatic development and β-cell proliferation.(PMID: 20119533/PMID: 27458237)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SPATA2 antibody 29321-1-AP | Download protocol |
| IHC protocol for SPATA2 antibody 29321-1-AP | Download protocol |
| WB protocol for SPATA2 antibody 29321-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
