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Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using SPATA13 Polyclonal antibody (21400-1-AP)

    WB analysis of COLO 320 using 21400-1-AP (same clone as 21400-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 21400-1-AP (SPATA13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21400-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using SPATA13 Polyclonal antibody (21400-1-AP)

    WB analysis of human placenta using 21400-1-AP (same clone as 21400-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 21400-1-AP (SPATA13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21400-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SPATA13 Polyclonal antibody (21400-1-AP)

    IF Staining of HepG2 using 21400-1-AP (same clone as 21400-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using SPATA13 antibody 21400-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 21400-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SPATA13 Polyclonal antibody (21400-1-AP)

    IF Staining of HeLa using 21400-1-AP (same clone as 21400-1-PBS)

    Immunofluorescent analysis of Hela cells, using SPATA13 antibody 21400-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 21400-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SPATA13 Polyclonal antibody (21400-1-AP)

    IF Staining of HEK-293 using 21400-1-AP (same clone as 21400-1-PBS)

    Immunofluorescent analysis of 293 cells, using SPATA13 antibody 21400-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 21400-1-PBS in a different storage buffer formulation.

SPATA13 Polyclonal antibody, PBS Only

SPATA13 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 21400-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21400-1-PBS targets SPATA13 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16101

Product name: Recombinant human SPATA13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-135 aa of BC109291

Sequence: MTSASPEDQNAPVGCPKGARRRRPISVIGGVSLYGTNQTEELDNLLTQPASRPPMPAHQVPPYKAVSARFRPFTFSQSTPIGLDRVGRRRQMRASNVSSDGGTEPSALVDDNGSEEDFSYEDLCQASPRYLQPGG

相同性解析による交差性が予測される生物種
Full Name spermatogenesis associated 13
Calculated molecular weight 652 aa, 75 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC109291
Gene Symbol SPATA13
Gene ID (NCBI) 221178
RRIDAB_10859783
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96N96
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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