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Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using SPATA13 Polyclonal antibody (21400-1-AP)

    WB analysis of COLO 320 using 21400-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 21400-1-AP (SPATA13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using SPATA13 Polyclonal antibody (21400-1-AP)

    WB analysis of human placenta using 21400-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 21400-1-AP (SPATA13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using SPATA13 Polyclonal antibody (21400-1-AP)

    IHC staining of human rectal cancer using 21400-1-AP

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 21400-1-AP (SPATA13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SPATA13 Polyclonal antibody (21400-1-AP)

    IHC staining of mouse kidney using 21400-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21400-1-AP (SPATA13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using SPATA13 Polyclonal antibody (21400-1-AP)

    IHC staining of mouse small intestine using 21400-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21400-1-AP (SPATA13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using SPATA13 Polyclonal antibody (21400-1-AP)

    IHC staining of rat kidney using 21400-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 21400-1-AP (SPATA13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using SPATA13 Polyclonal antibody (21400-1-AP)

    IHC staining of rat small intestine using 21400-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 21400-1-AP (SPATA13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SPATA13 Polyclonal antibody (21400-1-AP)

    IF Staining of HepG2 using 21400-1-AP

    Immunofluorescent analysis of HepG2 cells, using SPATA13 antibody 21400-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SPATA13 Polyclonal antibody (21400-1-AP)

    IF Staining of HeLa using 21400-1-AP

    Immunofluorescent analysis of Hela cells, using SPATA13 antibody 21400-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SPATA13 Polyclonal antibody (21400-1-AP)

    IF Staining of HEK-293 using 21400-1-AP

    Immunofluorescent analysis of 293 cells, using SPATA13 antibody 21400-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

SPATA13 Polyclonal antibody

SPATA13 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21400-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Asef2, spermatogenesis associated 13, Spermatogenesis-associated protein 13

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOLO 320 cells, human placenta tissue
Positive IHC detected inhuman rectal cancer tissue, mouse small intestine tissue, mouse kidney tissue, rat kidney tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HEK-293 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21400-1-AP targets SPATA13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16101

Product name: Recombinant human SPATA13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-135 aa of BC109291

Sequence: MTSASPEDQNAPVGCPKGARRRRPISVIGGVSLYGTNQTEELDNLLTQPASRPPMPAHQVPPYKAVSARFRPFTFSQSTPIGLDRVGRRRQMRASNVSSDGGTEPSALVDDNGSEEDFSYEDLCQASPRYLQPGG

相同性解析による交差性が予測される生物種
Full Name spermatogenesis associated 13
Calculated molecular weight 652 aa, 75 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC109291
Gene Symbol SPATA13
Gene ID (NCBI) 221178
RRIDAB_10859783
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96N96
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for SPATA13 antibody 21400-1-APDownload protocol
WB protocol for SPATA13 antibody 21400-1-APDownload protocol
IF protocol for SPATA13 antibody 21400-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Acta Biochim Biophys Sin (Shanghai)

Targeting the Spata13-TGFβRI interaction inhibits infection-driven capsular contracture via suppression of fibroblast activation.

Authors - Yiyin Tang
View Article
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