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Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using SPAG4-Specific Polyclonal antibody (19721-1-AP)

    WB analysis of human testis using 19721-1-AP (same clone as 19721-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 19721-1-AP (SPAG4-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SPAG4-Specific Polyclonal antibody (19721-1-AP)

    WB analysis of HepG2 using 19721-1-AP (same clone as 19721-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19721-1-AP (SPAG4-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using SPAG4-Specific Polyclonal antibody (19721-1-AP)

    WB analysis of human testis using 19721-1-AP (same clone as 19721-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 19721-1-AP (SPAG4-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SPAG4-Specific Polyclonal antibody (19721-1-AP)

    IHC staining of mouse testis using 19721-1-AP (same clone as 19721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19721-1-AP (SPAG4-Specific antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SPAG4-Specific Polyclonal antibody (19721-1-AP)

    IHC staining of mouse testis using 19721-1-AP (same clone as 19721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19721-1-AP (SPAG4-Specific antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using SPAG4-Specific Polyclonal antibody (19721-1-AP)

    IF Staining of mouse testis using 19721-1-AP (same clone as 19721-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SPAG4-Specific antibody (19721-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SPAG4-Specific Polyclonal antibody (19721-1-AP)

    IF Staining of HepG2 using 19721-1-AP (same clone as 19721-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 19721-1-AP (SPAG4-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

SPAG4-Specific Polyclonal antibody, PBS Only

SPAG4-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 19721-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19721-1-PBS targets SPAG4-Specific in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name sperm associated antigen 4
Calculated molecular weight 48 kDa
Observed molecular weight 55 kDa
GenBank accession numberNM_003116
Gene Symbol SPAG4
Gene ID (NCBI) 6676
RRIDAB_2878600
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NPE6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SPAG4 may assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail. This antibody is specific to SPAG4. We observed that the molecular weight of SPAG4-Specific was 55 kDa.

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