Validation Data Gallery

Filter: AllWBIHCIF-PIF/ICC

WB analysis of human testis using 19721-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 19721-1-AP (SPAG4-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 19721-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 19721-1-AP (SPAG4-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 19721-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 19721-1-AP (SPAG4-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 19721-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19721-1-AP (SPAG4-Specific antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 19721-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19721-1-AP (SPAG4-Specific antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse testis using 19721-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SPAG4-Specific antibody (19721-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 19721-1-AP
Immunofluorescent analysis of HepG2 cells using 19721-1-AP (SPAG4-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SPAG4-Specific Polyclonal antibody

SPAG4-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 19721-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

SPAG4, Outer dense fiber-associated protein SPAG4, SUN4, SUN domain-containing protein 4, Sperm-associated antigen 4 protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human testis tissue, HepG2 cells
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse testis tissue
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IHC	See 1 publications below

Product Information

19721-1-AP targets SPAG4-Specific in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	sperm associated antigen 4
Calculated molecular weight	48 kDa
Observed molecular weight	55 kDa
GenBank accession number	NM_003116
Gene Symbol	SPAG4
Gene ID (NCBI)	6676
RRID	AB_2878600
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NPE6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SPAG4 may assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail. This antibody is specific to SPAG4. We observed that the molecular weight of SPAG4-Specific was 55 kDa.

Protocols

Product Specific Protocols
IF protocol for SPAG4-Specific antibody 19721-1-AP	Download protocol
IHC protocol for SPAG4-Specific antibody 19721-1-AP	Download protocol
WB protocol for SPAG4-Specific antibody 19721-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Biol Chem The testis-specific LINC component SUN3 is essential for sperm head shaping during mouse spermiogenesis. Authors - Qian Gao View Article
human	WB	Cancer Cell Int Novel risk model based on angiogenesis-related lncRNAs for prognosis prediction of hepatocellular carcinoma Authors - Shicheng Xie View Article
human	WB	J Biochem Mol Toxicol SPAG4 enhances mitochondrial respiration and aerobic glycolysis in colorectal cancer cells by activating the PI3K/Akt signaling pathway Authors - Jiehao Zhou View Article
human	WB,IHC	BMC Med Genomics m6A regulator-based molecular classification and hub genes associated with immune infiltration characteristics and clinical outcomes in diffuse gliomas Authors - Jie Lu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human testis using 19721-1-AP

WB analysis of HepG2 using 19721-1-AP

WB analysis of human testis using 19721-1-AP

IHC staining of mouse testis using 19721-1-AP

IHC staining of mouse testis using 19721-1-AP

IF Staining of mouse testis using 19721-1-AP

IF Staining of HepG2 using 19721-1-AP