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Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using SPAG11A/B Polyclonal antibody (16770-1-AP)

    WB analysis of human testis using 16770-1-AP (same clone as 16770-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 16770-1-AP (SPAG11A/B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using SPAG11A/B Polyclonal antibody (16770-1-AP)

    WB analysis of human brain using 16770-1-AP (same clone as 16770-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 16770-1-AP (SPAG11A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using SPAG11A/B Polyclonal antibody (16770-1-AP)

    IHC staining of human testis using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 16770-1-AP (SPAG11A/B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using SPAG11A/B Polyclonal antibody (16770-1-AP)

    IHC staining of human testis using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 16770-1-AP (SPAG11A/B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SPAG11A/B Polyclonal antibody (16770-1-AP)

    IHC staining of human kidney using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16770-1-AP (SPAG11A/B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SPAG11A/B Polyclonal antibody (16770-1-AP)

    IHC staining of human kidney using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16770-1-AP (SPAG11A/B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SPAG11A/B Polyclonal antibody (16770-1-AP)

    IHC staining of human placenta using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 16770-1-AP (SPAG11A/B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SPAG11A/B Polyclonal antibody (16770-1-AP)

    IHC staining of human placenta using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 16770-1-AP (SPAG11A/B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

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SPAG11A/B Polyclonal antibody, PBS Only

SPAG11A/B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16770-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

SPAG11A, Antimicrobial-like protein Bin-1b, BIN1B, EP2, He2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16770-1-PBS targets SPAG11A/B in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPAG11A/B fusion protein Ag9846 相同性解析による交差性が予測される生物種
Full Name sperm associated antigen 11A
Calculated molecular weight 123 aa, 14 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC058833
Gene Symbol SPAG11A
Gene ID (NCBI) 653423
RRIDAB_2286582
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6PDA7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Sperm binding proteins and their C-terminal peptides of the Sperm Associated Antigen 11 (SPAG11) family are found to play an important role in epididymal innate immunity in addition to their role in sperm maturation(PMID:16643671). SPAG11 peptides resulting due to cleavage at the furin-like proprotein convertase motif are reported in the epididymal fluid, ejaculate and on sperm and their antimicrobial activity demonstrated.SPAG11A belongs to this family and is also named as EP2,HE2. It has 4 isoforms produced by alternative splicing. This antibody recognizes SPAG11A and SPAG11B, but not SPAG11, because SPAG11 is a different transcript.