Validation Data Gallery
Tested Applications
| Positive WB detected in | human testis tissue, human brain tissue |
| Positive IHC detected in | human testis tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
16770-1-AP targets SPAG11A in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SPAG11A fusion protein Ag9846 相同性解析による交差性が予測される生物種 |
| Full Name | sperm associated antigen 11A |
| Calculated molecular weight | 123 aa, 14 kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | BC058833 |
| Gene Symbol | SPAG11A |
| Gene ID (NCBI) | 653423 |
| RRID | AB_2286582 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6PDA7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sperm binding proteins and their C-terminal peptides of the Sperm AssociatedAntigen 11 (SPAG11) family are found to play an important role in epididymal innate immunity in addition to their role in sperm maturation(PMID:16643671).SPAG11 peptides resulting due to cleavage at the furin-like proprotein convertase motif are reported in the epididymal fluid, ejaculate and on sperm and their antimicrobial activity demonstrated.SPAG11A belongs to this family and is also named as EP2,HE2. It has 4 isoforms produced by alternative splicing.This antibody is specific to SPAG11.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SPAG11A antibody 16770-1-AP | Download protocol |
| IHC protocol for SPAG11A antibody 16770-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Reprod Biol Endocrinol Sperm-associated antigen 11A is expressed exclusively in the principal cells of the mouse caput epididymis in an androgen-dependent manner. |