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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using SP2 Polyclonal antibody (25000-1-AP)

    WB analysis of K-562 using 25000-1-AP (same clone as 25000-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 25000-1-AP (SP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25000-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using SP2 Polyclonal antibody (25000-1-AP)

    WB analysis of Jurkat using 25000-1-AP (same clone as 25000-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25000-1-AP (SP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25000-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using SP2 Polyclonal antibody (25000-1-AP)

    WB analysis of C6 using 25000-1-AP (same clone as 25000-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 25000-1-AP (SP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25000-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SP2 Polyclonal antibody (25000-1-AP)

    WB analysis of HEK-293 using 25000-1-AP (same clone as 25000-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25000-1-AP (SP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25000-1-PBS in a different storage buffer formulation.

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SP2 Polyclonal antibody, PBS Only

SP2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 25000-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25000-1-PBS targets SP2 in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SP2 fusion protein Ag8060 相同性解析による交差性が予測される生物種
Full Name Sp2 transcription factor
Calculated molecular weight 65 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC016680
Gene Symbol SP2
Gene ID (NCBI) 6668
RRIDAB_2879839
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02086
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SP2 also named as Sp2 transcription factor or KIAA0048 is a 613 amino acid protein, which contains 3 C2H2-type zinc fingers and belongs to the sp1 C2H2-type zinc finger protein family. SP2 binds to box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites in nucleus . SP2 may regulate expression of the T-cell antigen receptor (TCR) gene.