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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using SP2 Polyclonal antibody (25000-1-AP)

    WB analysis of Jurkat using 25000-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25000-1-AP (SP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using SP2 Polyclonal antibody (25000-1-AP)

    WB analysis of K-562 using 25000-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25000-1-AP (SP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using SP2 Polyclonal antibody (25000-1-AP)

    WB analysis of C6 using 25000-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 25000-1-AP (SP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SP2 Polyclonal antibody (25000-1-AP)

    WB analysis of HEK-293 using 25000-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25000-1-AP (SP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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SP2 Polyclonal antibody

SP2 Polyclonal Antibody for WB, ELISA
Cat No. 25000-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

KIAA0048, SP2, Sp2 transcription factor, Transcription factor Sp2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, K-562 cells, C6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

25000-1-AP targets SP2 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SP2 fusion protein Ag8060 相同性解析による交差性が予測される生物種
Full Name Sp2 transcription factor
Calculated molecular weight 65 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC016680
Gene Symbol SP2
Gene ID (NCBI) 6668
RRIDAB_2879839
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02086
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SP2 also named as Sp2 transcription factor or KIAA0048 is a 613 amino acid protein, which contains 3 C2H2-type zinc fingers and belongs to the sp1 C2H2-type zinc finger protein family. SP2 binds to box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites in nucleus . SP2 may regulate expression of the T-cell antigen receptor (TCR) gene.

Protocols

Product Specific Protocols
WB protocol for SP2 antibody 25000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Commun Biol

ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3Gal1) synthesis of Siglec ligands mediates anti-tumour immunity in prostate cancer

Authors - Rebecca Garnham
View Article