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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SOX5 Recombinant monoclonal antibody (86619-2-RR)

    WB analysis using 86619-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86619-2-RR (SOX5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-87 MG cells using SOX5 Recombinant monoclonal antibody (86619-2-RR)

    WB analysis of U-87 MG using 86619-2-RR

    WB result of SOX5 antibody (86619-2-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOX5 transfected U-87 MG cells.

  • Western Blot (WB) analysis of mouse brain tissue using SOX5 Recombinant monoclonal antibody (86619-2-RR)

    WB analysis of mouse brain using 86619-2-RR

    Mouse brain tissue were subjected to SDS PAGE followed by western blot with 86619-2-RR (SOX5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SOX5 Recombinant monoclonal antibody (86619-2-RR)

    IF Staining of HepG2 using 86619-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SOX5 antibody (86619-2-RR, Clone: 251512D11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using SOX5 Recombinant monoclonal antibody (86619-2-RR)

    IF Staining of A549 using 86619-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SOX5 antibody (86619-2-RR, Clone: 251512D11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • KD/KO Validated

SOX5 Recombinant monoclonal antibody

SOX5 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86619-2-RR
Clone No.251512D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

L SOX5, Transcription factor SOX-5

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, mouse brain tissue, U-87 MG cells, NCI-H1299 cells
Positive IF/ICC detected inHepG2 cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86619-2-RR targets SOX5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag4092

Product name: Recombinant human SOX5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-376 aa of BC029220

Sequence: DEVAQPLNLSAKPKTSDGKSPTSPTSPHMPALRINSGAGPLKASVPAALASPSARVSTIGYLNDHDAVTKAIQEARQMKEQLRREQQVLDGKVAVVNSLGLNNCRTEKEKTTLESLTQQLAVKQNEEGKFSHAMMDFNLSGDSDGSAGVSESRIYRESRGRGSNEPHIKRPMNAFMVWAKDERRKILQAFPDMHNSNISKILGSRWKAMTNLEKQPYYEEQARLSKQHLEKYPDYKYKPRPKRTCLVDGKKLRIGEYKAIMRNRRQEMRQYFNVGQQAQIPIATAGVVYPGAIAMAGMPSPHLPSEHSSVSSSPEPGMPVIQSTYGVKGEEPHIKEEIQAEDINGEIYDEYDEEEDDPDVDYGSDSENHIAGQAN

相同性解析による交差性が予測される生物種
Full Name SRY (sex determining region Y)-box 5
Calculated molecular weight 763 aa, 84 kDa
Observed molecular weight84-90 kDa
GenBank accession numberBC029220
Gene Symbol SOX5
Gene ID (NCBI) 6660
RRIDAB_3745013
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP35711
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SOX5 is a member of the SRY-related HMG-box (SOX) family of transcription factors, which play critical roles in cell fate determination and differentiation during embryonic development. SOX5 contains a highly conserved high-mobility group (HMG) DNA-binding domain that enables it to regulate target gene expression by bending DNA and facilitating chromatin remodeling. SOX5 is essential for chondrogenesis, neurogenesis, and gliogenesis, and its dysregulation has been implicated in various cancers and developmental disorders (PMID: 19647094, PMID: 30914279). SOX5 has two known protein isoforms: a short isoform of 48 kDa and a long isoform of 84 kDa (PMID: 21732189). This antibody detects the 84-90 kDa band and has passed KD/KO validation.

Protocols

Product Specific Protocols
IF protocol for SOX5 antibody 86619-2-RRDownload protocol
WB protocol for SOX5 antibody 86619-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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