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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SOX5 Recombinant monoclonal antibody (86619-2-RR)

    WB analysis using 86619-2-RR (same clone as 86619-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86619-2-RR (SOX5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86619-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-87 MG cells using SOX5 Recombinant monoclonal antibody (86619-2-RR)

    WB analysis of U-87 MG using 86619-2-RR (same clone as 86619-2-PBS)

    WB result of SOX5 antibody (86619-2-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOX5 transfected U-87 MG cells. This data was developed using the same antibody clone with 86619-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using SOX5 Recombinant monoclonal antibody (86619-2-RR)

    WB analysis of mouse brain using 86619-2-RR (same clone as 86619-2-PBS)

    Mouse brain tissue were subjected to SDS PAGE followed by western blot with 86619-2-RR (SOX5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86619-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SOX5 Recombinant monoclonal antibody (86619-2-RR)

    IF Staining of HepG2 using 86619-2-RR (same clone as 86619-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SOX5 antibody (86619-2-RR, Clone: 251512D11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86619-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using SOX5 Recombinant monoclonal antibody (86619-2-RR)

    IF Staining of A549 using 86619-2-RR (same clone as 86619-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SOX5 antibody (86619-2-RR, Clone: 251512D11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86619-2-PBS in a different storage buffer formulation.

SOX5 Recombinant monoclonal antibody, PBS Only

SOX5 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86619-2-PBS
Clone No.251512D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

L SOX5, Transcription factor SOX-5

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86619-2-PBS targets SOX5 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag4092

Product name: Recombinant human SOX5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-376 aa of BC029220

Sequence: DEVAQPLNLSAKPKTSDGKSPTSPTSPHMPALRINSGAGPLKASVPAALASPSARVSTIGYLNDHDAVTKAIQEARQMKEQLRREQQVLDGKVAVVNSLGLNNCRTEKEKTTLESLTQQLAVKQNEEGKFSHAMMDFNLSGDSDGSAGVSESRIYRESRGRGSNEPHIKRPMNAFMVWAKDERRKILQAFPDMHNSNISKILGSRWKAMTNLEKQPYYEEQARLSKQHLEKYPDYKYKPRPKRTCLVDGKKLRIGEYKAIMRNRRQEMRQYFNVGQQAQIPIATAGVVYPGAIAMAGMPSPHLPSEHSSVSSSPEPGMPVIQSTYGVKGEEPHIKEEIQAEDINGEIYDEYDEEEDDPDVDYGSDSENHIAGQAN

相同性解析による交差性が予測される生物種
Full Name SRY (sex determining region Y)-box 5
Calculated molecular weight 763 aa, 84 kDa
Observed molecular weight84-90 kDa
GenBank accession numberBC029220
Gene Symbol SOX5
Gene ID (NCBI) 6660
RRIDAB_3745013
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP35711
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SOX5 is a member of the SRY-related HMG-box (SOX) family of transcription factors, which play critical roles in cell fate determination and differentiation during embryonic development. SOX5 contains a highly conserved high-mobility group (HMG) DNA-binding domain that enables it to regulate target gene expression by bending DNA and facilitating chromatin remodeling. SOX5 is essential for chondrogenesis, neurogenesis, and gliogenesis, and its dysregulation has been implicated in various cancers and developmental disorders (PMID: 19647094, PMID: 30914279). SOX5 has two known protein isoforms: a short isoform of 48 kDa and a long isoform of 84 kDa (PMID: 21732189). This antibody detects the 84-90 kDa band and has passed KD/KO validation.

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