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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX25 Polyclonal antibody (13294-1-AP)

    WB analysis using 13294-1-AP (same clone as 13294-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13294-1-AP (SNX25 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13294-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SNX25 Polyclonal antibody (13294-1-AP)

    WB analysis using 13294-1-AP (same clone as 13294-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13294-1-AP (SNX25 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13294-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using SNX25 Polyclonal antibody (13294-1-AP)

    WB analysis of Jurkat using 13294-1-AP (same clone as 13294-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13294-1-AP (SNX25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13294-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using SNX25 Polyclonal antibody (13294-1-AP)

    WB analysis of mouse thymus using 13294-1-AP (same clone as 13294-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13294-1-AP (SNX25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13294-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Raji cells using SNX25 Polyclonal antibody (13294-1-AP)

    IP experiment of Raji using 13294-1-AP (same clone as 13294-1-PBS)

    IP result of anti-SNX25 (IP:13294-1-AP, 4ug; Detection:13294-1-AP 1:2000) with Raji cells lysate 1600 ug. This data was developed using the same antibody clone with 13294-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNX25 Polyclonal antibody (13294-1-AP)

    IF Staining of HeLa using 13294-1-AP (same clone as 13294-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SNX25 antibody (13294-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 13294-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SNX25 Polyclonal antibody, PBS Only

SNX25 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 13294-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Sorting nexin-25, sorting nexin 25, SBBI31, MSTP043

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13294-1-PBS targets SNX25 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNX25 fusion protein Ag3995 相同性解析による交差性が予測される生物種
Full Name sorting nexin 25
Calculated molecular weight 98 kDa
Observed molecular weight 98-105 kDa, 65 kDa
GenBank accession numberBC029868
Gene Symbol SNX25
Gene ID (NCBI) 83891
RRIDAB_2192549
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H3E2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SNXs (sorting nexin), a family of proteins playing roles in cargo sorting and signaling from compartments within the endocytic network, regulate traffic of membrane proteins including TGF-β receptors. Sorting Nexin 25 (SNX25) has been recently proposed to modulate TGF-β signaling through endosomal sorting of TGF-β receptors for lysosomal degradation.This antibody can recognize two isoforms: 98-105 kDa and 65 kDa.