Validation Data Gallery
Tested Applications
| Positive WB detected in | EL-4 cells, Raji cells, Jurkat cells, mouse thymus tissue, C6 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | Raji cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
13294-1-AP targets SNX25 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3995 Product name: Recombinant human SNX25 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 207-556 aa of BC029868 Sequence: QINEQASFAVNKLRELNEKLEYKRQALNSIQNAPKPDKKIVSKLKDEIILIEKERTDLQLHMARTDWWCENLGMWKASITSGEVTEENGEQLPCYFVMVSLQEVGGVETKNWTVPRRLSEFQNLHRKLSECVPSLKKVQLPSLSKLPFKSIDQKFMEKSKNQLNKFLQEETEEDSDLSDYGDDVDGRKDALAEPCFMLIGEIFELRGMFKWVRRTLIALVQVTFGRTINKQIRDTVSWIFSEQMLVYYINIFRDAFWPNGKLAPPTTIRSKEQSQETKQRAQQKLLENIPDMLQSLVGQQNARHGIIKIFNALQETRANKHLLYALMELLLIELCPELRVHLDQLKAGQV 相同性解析による交差性が予測される生物種 |
| Full Name | sorting nexin 25 |
| Calculated molecular weight | 98 kDa |
| Observed molecular weight | 98-105 kDa, 65 kDa |
| GenBank accession number | BC029868 |
| Gene Symbol | SNX25 |
| Gene ID (NCBI) | 83891 |
| RRID | AB_2192549 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H3E2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SNXs (sorting nexin), a family of proteins playing roles in cargo sorting and signaling from compartments within the endocytic network, regulate traffic of membrane proteins including TGF-β receptors. Sorting Nexin 25 (SNX25) has been recently proposed to modulate TGF-β signaling through endosomal sorting of TGF-β receptors for lysosomal degradation.This antibody can recognize two isoforms: 98-105 kDa and 65 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SNX25 antibody 13294-1-AP | Download protocol |
| IP protocol for SNX25 antibody 13294-1-AP | Download protocol |
| WB protocol for SNX25 antibody 13294-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Immunol Dermal macrophages set pain sensitivity by modulating the amount of tissue NGF through an SNX25-Nrf2 pathway | ||
Redox Biol Redox-modulated SNX25 as a novel regulator of GPCR-G protein signaling from endosomes | ||
Sci Rep Dermal macrophages control tactile perception under physiological conditions via NGF signaling | ||
Brain Struct Funct Neural expression of sorting nexin 25 and its regulation of tyrosine receptor kinase B trafficking. | ||
PLoS One SNX25 regulates proinflammatory cytokine expression via the NF-κB signal in macrophages.
| ||
Neurosci Lett Circadian rhythms of sorting nexin 25 in the mouse suprachiasmatic nucleus. |